mouse albumin enzyme linked immunosorbent assay quantitation kit  (Cusabio)

Journal: Cell Death Discovery

Article Title: IRF-8/miR-451a regulates M-MDSC differentiation via the AMPK/mTOR signal pathway during lupus development

doi: 10.1038/s41420-021-00568-z

Figure Lengend Snippet: BALB/c mice (10 weeks, n = 6–8/group) were given a single intraperitoneal injection of 0.5 ml pristane or PBS and monitored for 7 months. Metformin-treated lupus mice for 2 months after 5 months pristane injection. A Kidney sections from each group showed histologic differences. B Proteinuria in each group was determined using Mouse Albumin ELISA Quantitation Set. C Serum levels of total IgG and IgG against dsDNA were determined by ELISA. D Lung sections from each group showed histologic differences. E Representative photographs of paws from each group. F – K Gr1mAb, M-MDSCs or Metformin-treated lupus M-MDSCs treated mice for 1 month. F Kidney sections from each group showed histologic differences. G Serum levels of total IgG and IgG against dsDNA were determined by ELISA Quantitation Set. H Proteinuria in each group was determined using mouse albumin. I , K Lung sections from each group showed histologic differences. J , K Representative histological sections of tarsal hind paw joints. Data represent the mean ± SEM. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001.

Article Snippet: Mouse albumin enzyme linked immunosorbent assay (ELISA) quantitation set, mouse anti-IgG, and anti-dsDNA IgG kit were purchased from Bethyl Laboratories Inc.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Quantitation Assay

Journal: Scientific Reports

Article Title: MC1R is dispensable for the proteinuria reducing and glomerular protective effect of melanocortin therapy

doi: 10.1038/srep27589

Figure Lengend Snippet: Male MC1R e/e mice and wild-type (WT) littermates aged 8 weeks were treated with saline or lipopolysaccharide (LPS, 200 μg) intraperitoneally. One hour before and 12 h after LPS or saline injection, mice received subcutaneous injection of NDP-MSH (0.6 μmol/kg wt ) or an equal amount of saline. Mice were followed for 24 h. ( a ) Urine was collected and processed for urine albumin ELISA assay adjusted for urine creatinine concentrations. # P < 0.05 vs control group (n = 6); * P < 0.05 vs LPS group (n = 6). ( b ) Kidney cortical tissues were procured at 24 h and processed for transmission electron microscopy. LPS injury caused marked ultrastructural signs of podocytopathy, characterized by microvillous transformation and a variable degree of foot process effacement (black arrowheads). This effect was attenuated by NDP-MSH treatment equally in MC1R e/e mice and WT littermates. Scale bar = 1 μm. ( c ) Morphometric analysis of the number of foot processes per micrometer of glomerular basement membrane (GBM) by electron microscopy. # P < 0.01 vs control group (n = 6); * P < 0.05 vs LPS group (n = 6).

Article Snippet: Urine albumin concentration was measured using a mouse albumin enzyme-linked immunosorbent assay (ELISA) quantitation kit (Bethyl Laboratories Inc., Montgomery, TX).

Techniques: Saline, Injection, Enzyme-linked Immunosorbent Assay, Control, Transmission Assay, Electron Microscopy, Transformation Assay, Membrane